Using a powerful analytical technique known as ChemoMorphic Analysis, INTiDYN evaluates small biopsies from animals or humans to create a morphological, biochemical, and genomic snapshot of gender, age, disease, drug, or toxin impacts in complex tissue. Interpretation of these analyses yields critical information about tissue transformations and molecular/cellular interactions, as well as the effects on the various functional systems integrated within the skin (e.g., cardiovascular, nervous, endocrine, immunological).

CMA

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a
Clinical Assessment and Skin Biopsy

Clinical sensory testing is performed, followed by the collection of 3mm skin biopsies which are immediately immersion fixed or flash frozen.
b
Biopsy Sectioning and Sequential Distribution

Immersion fixed and flash frozen biopsies are cut into sections and sequentially mounted onto a series of slides for immunochemistry or laser capture. This method of tissue distribution ensures that each slide contains sections throughout the biopsy. A mix of section thicknesses and number of sections per slide optimizes tissue use and maximizes options for biomarker combinations.
c
Multimolecular Immunolabeling

Individual slides are processed for immunofluorescent detection of protein expression using a variety of antibody combinations – those desired by the client and/or recommended by INTiDYN .
d
Compartmentalized Qualitative and Quantitative Microscopic Analysis

Immunolabeled slides are evaluated under epifluorescence wide-field and confocal microscopy. Individual tissue compartments undergo qualitative assessments and quantitative measures of endpoints chosen by our clients and/or recommended by INTiDYN .
e
Compartmentalized Laser Capture Microdissection

Flash frozen sections are collected on slides for laser capture microdissection (LCM) of individual skin compartments, such as epidermis. Compartment specific etchings from sections throughout the biopsy are pooled for nucleic acid extraction.
f
Nucleic Acid Extraction and Functional Genomic Assays

Total genomic DN A (gDN A), micro RNA (miRNA), and messenger RNA (mRNA) are recovered for array analysis or quantitative PCR (qPCR) to the specifications of our clients and/or at the recommendation of INTiDYN .
g
Data Compilation

INTiDYN scientists utilize all levels of data generated within the scope of a project (clinical, cellular/molecular, genetic) to determine:

Potential disease mechanisms and therapeutic targets

Efficacy and/or off-target toxicity of potential therapeutics

Impacts of normal demographic variables such as gender, race, and age on disease mechanisms and potential therapeutics